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WB analysis of various sample lysates using GTX16389 Tyrosinase antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX16389 Tyrosinase antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX16389 Tyrosinase antibody. Dilution : 1:1000 Loading : 25microg per lane

Tyrosinase antibody

GTX16389
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTYR
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Overview

  • Supplier
    GeneTex
  • Product Name
    Tyrosinase antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. IHC-P: 1:50 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7299
  • Target name
    TYR
  • Target description
    tyrosinase
  • Target synonyms
    ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3, tyrosinase, LB24-AB, SK29-AB, monophenol monooxygenase, oculocutaneous albinism IA, tumor rejection antigen AB
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14679
  • Protein Name
    Tyrosinase
  • Scientific Description
    The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3 half of this gene. [provided by RefSeq, Oct 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Ścieżyńska A, Sobiepanek A, Kowalska PD, et al. A Novel and Effective Method for Human Primary Skin Melanocytes and Metastatic Melanoma Cell Isolation. Cancers (Basel). 2021,13(24). doi: 10.3390/cancers13246244
    Read this paper
  • Hseu YC, Vudhya Gowrisankar Y, Wang LW, et al. The in vitro and in vivo depigmenting activity of pterostilbene through induction of autophagy in melanocytes and inhibition of UVA-irradiated α-MSH in keratinocytes via Nrf2-mediated antioxidant pathways. Redox Biol. 2021,44:102007. doi: 10.1016/j.redox.2021.102007
    Read this paper
  • Lan Y, Zeng W, Dong X, et al. Opsin 5 is a key regulator of ultraviolet radiation-induced melanogenesis in human epidermal melanocytes. Br J Dermatol. 2021,185(2):391-404. doi: 10.1111/bjd.19797
    Read this paper

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Figure 1. Western blot analysis of Tyrosinase/TYR using anti-Tyrosinase/TYR antibody (A00326-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A375 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tyrosinase/TYR antigen affinity purified polyclonal antibody (Catalog # A00326-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tyrosinase/TYR at approximately 60-65 kDa. The expected band size for Tyrosinase/TYR is at 60 kDa.
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