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ProductsBack/U1 small nuclear ribonucleoprotein A Polyclonal Conjugated Antibody

U1 small nuclear ribonucleoprotein A Polyclonal Conjugated Antibody

ORB1617995
Biorbyt
ReactivityHuman
Product group Antibodies
TargetSNRPA
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Overview

  • Supplier
    Biorbyt
  • Product Name
    U1 small nuclear ribonucleoprotein A Polyclonal Conjugated Antibody
  • Delivery Days Customer
    10
  • Applications Supplier
    working with enzyme-conjugated streptavidin, 1: 50 - 1: 1,000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Gene ID6626
  • Target name
    SNRPA
  • Target description
    small nuclear ribonucleoprotein polypeptide A
  • Target synonyms
    Mud1, U1-A, U1A, U1 small nuclear ribonucleoprotein A, U1 small nuclear RNP-specific A, U1 snRNP A, U1 snRNP-specific protein A
  • Host
    Rabbit
  • Protein IDP09012
  • Protein Name
    U1 small nuclear ribonucleoprotein A
  • Scientific Description
    U1 small nuclear ribonucleoprotein A Polyclonal Conjugated Antibody
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of U1A/SNRPA using anti-U1A/SNRPA antibody (A08780-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-U1A/SNRPA antigen affinity purified polyclonal antibody (Catalog # A08780-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for U1A/SNRPA at approximately 31 kDa. The expected band size for U1A/SNRPA is at 31 kDa.
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