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UBE2I / UBC9 Antibody

LS-C814043
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUBE2I
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UBE2I / UBC9 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:10000), Flo (1:200 - 1:400), IF (1:200 - 1:1000), IHC (1:200 - 1:1000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7329
  • Target name
    UBE2I
  • Target description
    ubiquitin conjugating enzyme E2 I
  • Target synonyms
    C358B7.1, P18, UBC9, SUMO-conjugating enzyme UBC9, RING-type E3 SUMO transferase UBC9, SUMO-1-protein ligase, SUMO-protein ligase, ubiquitin carrier protein 9, ubiquitin carrier protein I, ubiquitin conjugating enzyme 9, ubiquitin conjugating enzyme E2I, ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast), ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-conjugating enzyme UbcE2A, ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitin-protein ligase E2I, ubiquitin-protein ligase I
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of UBC9/UBE2I using anti-UBC9/UBE2I antibody (A02295-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBC9/UBE2I antigen affinity purified polyclonal antibody (A02295-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBC9/UBE2I at approximately 18 kDa. The expected band size for UBC9/UBE2I is at 18 kDa.
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