IHC image of CSB-PA025470LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IHC image of CSB-PA025470LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
UBE2N Antibody
CSB-PA025470LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBE2N
Overview
- SupplierCusabio
- Product NameUBE2N Antibody
- Delivery Days Customer20
- ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- ConjugateUnconjugated
- Gene ID7334
- Target nameUBE2N
- Target descriptionubiquitin conjugating enzyme E2 N
- Target synonymsbendless-like ubiquitin conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; HEL-S-71; UBC13; UbcH13; UbcH-ben; UBCHBEN; UBC13; ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-protein ligase N; yeast UBC13 homolog
- HostRabbit
- IsotypeIgG
- Protein IDP61088
- Protein NameUbiquitin-conjugating enzyme E2 N
- Scientific DescriptionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical \\\Lys-63\\\-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the \\\Lys-63\\\-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the \\\Lys-63\\\-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate \\\Lys-63\\\-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes.
- ReactivityHuman
- Storage Instruction-20°C or -80°C
- UNSPSC12352203