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UBE2N Polyclonal Antibody

CAC13238
Biomatik
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUBE2N
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Overview

  • Supplier
    Biomatik
  • Product Name
    UBE2N Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7334
  • Target name
    UBE2N
  • Target description
    ubiquitin conjugating enzyme E2 N
  • Target synonyms
    bendless-like ubiquitin conjugating enzyme; E2 ubiquitin-conjugating enzyme N; epididymis secretory protein Li 71; HEL-S-71; UBC13; UbcH13; UbcH-ben; UBCHBEN; UBC13; ubiquitin carrier protein N; ubiquitin conjugating enzyme E2N; ubiquitin-conjugating enzyme E2 N; ubiquitin-conjugating enzyme E2N (homologous to yeast UBC13); ubiquitin-conjugating enzyme E2N (UBC13 homolog, yeast); ubiquitin-protein ligase N; yeast UBC13 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP61088
  • Protein Name
    Ubiquitin-conjugating enzyme E2 N
  • Scientific Description
    The UBE2N Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, IHC, IF.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ubc13/UBE2N using anti-Ubc13/UBE2N antibody (A02462-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human MOLT-4 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: rat thymus tissue lysates, Lane 7: rat C6 whole cell lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse small intestine tissue lysates, Lane 10: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ubc13/UBE2N antigen affinity purified polyclonal antibody (Catalog # A02462-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ubc13/UBE2N at approximately 17 kDa. The expected band size for Ubc13/UBE2N is at 17 kDa.
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IHC image of CSB-PA025470LA01HU diluted at 1:200 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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