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Western blot All lanes: UBE2Q2 antibody at 2microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 37, 41, 39 kDa Observed band size: 43 kDa
Western blot All lanes: UBE2Q2 antibody at 2microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 37, 41, 39 kDa Observed band size: 43 kDa
Western blot All lanes: UBE2Q2 antibody at 2microg/ml + A431 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 43, 37, 41, 39 kDa Observed band size: 43 kDa

UBE2Q2 Antibody

CSB-PA845153HA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetUBE2Q2
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Overview

  • Supplier
    Cusabio
  • Product Name
    UBE2Q2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID92912
  • Target name
    UBE2Q2
  • Target description
    ubiquitin conjugating enzyme E2 Q2
  • Target synonyms
    ubiquitin-conjugating enzyme E2 Q2, E2 ubiquitin-conjugating enzyme Q2, ubiquitin carrier protein Q2, ubiquitin conjugating enzyme E2Q family member 2, ubiquitin-protein ligase Q2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WVN8
  • Protein Name
    Ubiquitin-conjugating enzyme E2 Q2
  • Scientific Description
    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes Lys-48-linked polyubiquitination.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBE2Q2 using anti-UBE2Q2 antibody (PB9839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: A431 whole cell lysates, Lane 3: MCF-7 whole cell lysates, Lane 4: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Q2 antigen affinity purified polyclonal antibody (Catalog # PB9839) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2Q2 at approximately 50 kDa. The expected band size for UBE2Q2 is at 50 kDa.
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