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Anti-UBE2Q2 Antibody

CAB9992
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetUBE2Q2
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-UBE2Q2 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID92912
  • Target name
    UBE2Q2
  • Target description
    ubiquitin conjugating enzyme E2 Q2
  • Target synonyms
    ubiquitin-conjugating enzyme E2 Q2, E2 ubiquitin-conjugating enzyme Q2, ubiquitin carrier protein Q2, ubiquitin conjugating enzyme E2Q family member 2, ubiquitin-protein ligase Q2
  • Host
    Rabbit
  • Protein IDQ8WVN8
  • Protein Name
    Ubiquitin-conjugating enzyme E2 Q2
  • Scientific Description
    UBE2Q2 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human,Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of UBE2Q2 using anti-UBE2Q2 antibody (PB9839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: HELA whole cell lysates, Lane 2: A431 whole cell lysates, Lane 3: MCF-7 whole cell lysates, Lane 4: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBE2Q2 antigen affinity purified polyclonal antibody (Catalog # PB9839) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBE2Q2 at approximately 50 kDa. The expected band size for UBE2Q2 is at 50 kDa.
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