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Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)

Ubiquitin Activating Enzyme E1 [UBA1] (human) (rec.) (His)

SBB-CE0058
South Bay Bio
Protein IDP22314
Product group Proteins / Signaling Molecules
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Overview

  • Supplier
    South Bay Bio
  • Product Name
    Ubiquitin Activating Enzyme E1 [UBA1; UBE1] (human) (rec.) (His)
  • Delivery Days Customer
    10
  • Certification
    Research Use Only
  • Estimated Purity
    >95%
  • Protein IDP22314
  • Protein Name
    Ubiquitin-like modifier-activating enzyme 1
  • Scientific Description
    Protein. Human UBA1 (aa12-1058) is fused at the N-terminus to a His-tag. Source: Sf21 cells. Liquid. In 50mM HEPES pH 7.5, 150mM sodium chloride, 10% glycerol, 2mM TCEP. Purity: >95% (SDS-PAGE). UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzymes active site cysteine. - UBA1, the canonical ubiquitin E1 activating enzyme is a 118kDa protein, which forms a homodimer in its active state and catalyzes the first step of the ubiquitin conjugation cascade. It activates ubiquitin in an ATP-dependent mechanism where ATP is hydrolyzed to AMP and PPi, a ubiquitin C-terminal adenylate intermediate is formed, then transferred to the E1s active site cysteine through a thioester bond. This thioester is then transferable to an E2 conjugating enzymes active site cysteine.
  • Storage Instruction
    -80°C
  • UNSPSC
    12352202

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