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Ubiquitin antibody

GTX128826
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    GeneTex
  • Product Name
    Ubiquitin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.68 mg/ml
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • d'Angelo M, Brandolini L, Catanesi M, et al. Differential Effects of Nonsteroidal Anti-Inflammatory Drugs in an In Vitro Model of Human Leaky Gut. Cells. 2023,12(5). doi: 10.3390/cells12050728
    Read this paper
  • Ko CY, Lin CH, Chuang JY, et al. MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance. Mol Neurobiol. 2018,55(4):3211-3223. doi: 10.1007/s12035-017-0569-4
    Read this paper
  • Tsao SM, Hsu HY. Fucose-containing fraction of Ling-Zhi enhances lipid rafts-dependent ubiquitination of TGFβ receptor degradation and attenuates breast cancer tumorigenesis. Sci Rep. 2016,6:36563. doi: 10.1038/srep36563
    Read this paper
  • Hsu HY, Lin TY, Wu YC, et al. Fucoidan inhibition of lung cancer in vivo and in vitro : role of the Smurf2-dependent ubiquitin proteasome pathway in TGFβ receptor degradation. Oncotarget. 2014,5(17):7870-85.
    Read this paper

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Western blot of Ubiquitin. Anti-Ubiquitin antibody (GTX14372), generated by immunization with Ubiquitin coupled to Rabbit IgG, was tested by western blot against total cell extract from yeast. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity with Ubiquitinated proteins. In this blot the antibody was used at a 1:500 dilution incubated overnight at 4o C in 5% non-fat dry milk in TBST. Detection occurred using a 1:2,000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature.
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The data was published in the journal Front Oncol in 2019. PMID: 31024851
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