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UBR2 Antibody

LS-C830395
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetUBR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UBR2 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:40 - 1:250)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID23304
  • Target name
    UBR2
  • Target description
    ubiquitin protein ligase E3 component n-recognin 2
  • Target synonyms
    C6orf133, bA49A4.1, dJ242G1.1, dJ392M17.3, E3 ubiquitin-protein ligase UBR2, RING-type E3 ubiquitin transferase UBR2, ubiquitin-protein ligase E3-alpha-2, ubiquitin-protein ligase E3-alpha-II
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UBR2 using anti-UBR2 antibody (A05812-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: mouse RAW264.7 tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UBR2 antigen affinity purified polyclonal antibody (Catalog # A05812-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UBR2 at approximately 201KD. The expected band size for UBR2 is at 201KD.
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