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ProductsBack/UCHL1 / PGP9.5 Antibody

UCHL1 / PGP9.5 Antibody

LS-C813145
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetUCHL1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    UCHL1 / PGP9.5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:10000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7345
  • Target name
    UCHL1
  • Target description
    ubiquitin C-terminal hydrolase L1
  • Target synonyms
    epididymis luminal protein 117; epididymis secretory protein Li 53; HEL-117; HEL-S-53; NDGOA; neuron cytoplasmic protein 9.5; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L1; ubiquitin thioesterase L1; ubiquitin thiolesterase; Uch-L1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PGP9.5 using anti-PGP9.5 antibody (PB9840). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP9.5 antigen affinity purified polyclonal antibody (Catalog # PB9840) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PGP9.5 at approximately 25 kDa. The expected band size for PGP9.5 is at 25 kDa.
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