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UCP2 Polyclonal Antibody

RD70458A
Reddot Biotech
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUCP2
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Overview

  • Supplier
    Reddot Biotech
  • Product Name
    UCP2 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.62 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7351
  • Target name
    UCP2
  • Target description
    uncoupling protein 2
  • Target synonyms
    BMIQ4, SLC25A8, UCPH, dicarboxylate carrier SLC25A8, mitochondrial uncoupling protein 2, solute carrier family 25 member 8, uncoupling protein 2 (mitochondrial, proton carrier)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55851
  • Protein Name
    Dicarboxylate carrier SLC25A8
  • Scientific Description
    Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. - This is a UCP2 Polyclonal Antibody from Reddot Biotech. This product is for Research Use Only.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of UCP2 using anti-UCP2 antibody (A02256-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysate,Lane 2: rat cardiac muscle tissue lysate,Lane 3: rat brain tissue lysate,Lane 4: mouse spleen tissue lysate,Lane 5: mouse cardiac muscle tissue lysate,Lane 6: mouse brain tissue lysate,Lane 7: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCP2 antigen affinity purified polyclonal antibody (Catalog # A02256-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UCP2 at approximately 33KD. The expected band size for UCP2 is at 33KD.
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