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Immunohistochemistry of paraffin-embedded Rat Intestine using UHRF2 Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Rat Intestine using UHRF2 Polyclonal Antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Rat Intestine using UHRF2 Polyclonal Antibody at dilution of 1:200 (40x lens).

UHRF2 Polyclonal Antibody

E-AB-64743
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    UHRF2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    This gene encodes a nuclear protein which is involved in cell-cycle regulation. The encoded protein is a ubiquitin-ligase capable of ubiquinating PCNP (PEST-containing nuclear protein), and together they may play a role in tumorigenesis. The encoded protein contains an NIRF_N domain, a PHD finger, a set- and ring-associated (SRA) domain, and a RING finger domain and several of these domains have been shown to be essential for the regulation of cell proliferation. This protein may also have a role in intranuclear degradation of polyglutamine aggregates. Alternative splicing results in multiple transcript variants some of which are non-protein coding.
  • UNSPSC
    12352203

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The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using CSB-PA049538(UHRF2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
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Figure 1. Western blot analysis of NIRF using anti-NIRF antibody (PB9906). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NIRF antigen affinity purified polyclonal antibody (Catalog # PB9906) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NIRF at approximately 90 kDa. The expected band size for NIRF is at 90 kDa.
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