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ProductsBack/ULK3 Antibody - C-terminal region (OAAB17808)

ULK3 Antibody - C-terminal region (OAAB17808)

OAAB17808
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetULK3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    ULK3 Antibody - C-terminal region (OAAB17808)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Format
    Liquid. Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
  • Gene ID25989
  • Target name
    ULK3
  • Target description
    unc-51 like kinase 3
  • Target synonyms
    serine/threonine-protein kinase ULK3
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ULK3 using anti-ULK3 antibody (PB9519). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Rat Testis Tissue Lysate at 50ug, Lane 3: Mouse Brain Tissue Lysate at 50ug, Lane 4: Human Placenta Tissue Lysate at 50ug, Lane 5: 22RV1 Whole Cell Lysate at 40ug, Lane 6: SMMC Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ULK3 antigen affinity purified polyclonal antibody (Catalog # PB9519) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ULK3 at approximately 53 kDa. The expected band size for ULK3 is at 53 kDa.
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