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Western Blot analysis of COLO205 cells using UDG Polyclonal Antibody
Western Blot analysis of COLO205 cells using UDG Polyclonal Antibody
Western Blot analysis of COLO205 cells using UDG Polyclonal Antibody

UNG Antibody

CSB-PA004386
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUNG
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Overview

  • Supplier
    Cusabio
  • Product Name
    UNG Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7374
  • Target name
    UNG
  • Target description
    uracil DNA glycosylase
  • Target synonyms
    DGU, HIGM4, HIGM5, UDG, UNG1, UNG15, UNG2, uracil-DNA glycosylase, uracil-DNA glycosylase 1, uracil-DNA glycosylase 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP13051
  • Protein Name
    Uracil-DNA glycosylase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of UNG using anti-UNG antibody (A01672-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: monkey heart tissue lysates, Lane 6: rat heart tissue lysates, Lane 7: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UNG antigen affinity purified polyclonal antibody (Catalog # A01672-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UNG at approximately 35KD. The expected band size for UNG is at 35KD.
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