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UPF3A Antibody

CSB-PA025652GA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetUPF3A
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Overview

  • Supplier
    Cusabio
  • Product Name
    UPF3A Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65110
  • Target name
    UPF3A
  • Target description
    UPF3A regulator of nonsense mediated mRNA decay
  • Target synonyms
    hUpf3; HUPF3A; nonsense mRNA reducing factor 3A; regulator of nonsense transcripts 3A; RENT3A; UPF3; UPF3 regulator of nonsense transcripts homolog A; up-frameshift suppressor 3 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1J1
  • Protein Name
    Regulator of nonsense transcripts 3A
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UPF3A using anti-UPF3A antibody (A08388-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF3A antigen affinity purified polyclonal antibody (Catalog # A08388-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPF3A at approximately 51KD. The expected band size for UPF3A is at 51KD.
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