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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in developing spermatocytes.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in developing spermatocytes.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in developing spermatocytes.

Anti-UPF3A Antibody

HPA018325
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetUPF3A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-UPF3A
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65110
  • Target name
    UPF3A
  • Target description
    UPF3A regulator of nonsense mediated mRNA decay
  • Target synonyms
    hUpf3; HUPF3A; nonsense mRNA reducing factor 3A; regulator of nonsense transcripts 3A; RENT3A; UPF3; UPF3 regulator of nonsense transcripts homolog A; up-frameshift suppressor 3 homolog A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H1J1
  • Protein Name
    Regulator of nonsense transcripts 3A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of UPF3A using anti-UPF3A antibody (A08388-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat PC-12 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UPF3A antigen affinity purified polyclonal antibody (Catalog # A08388-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UPF3A at approximately 51KD. The expected band size for UPF3A is at 51KD.
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