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Western Blot analysis of 3T3 cells using USP16 Polyclonal Antibody
Western Blot analysis of 3T3 cells using USP16 Polyclonal Antibody
Western Blot analysis of 3T3 cells using USP16 Polyclonal Antibody

USP16 Antibody

CSB-PA006653
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUSP16
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Overview

  • Supplier
    Cusabio
  • Product Name
    USP16 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10600
  • Target name
    USP16
  • Target description
    ubiquitin specific peptidase 16
  • Target synonyms
    deubiquitinating enzyme 16; ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific protease 16; ubiquitin thioesterase 16; ubiquitin thiolesterase 16; ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; UBPM; UBP-M
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5T5
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 16
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of USP16 using anti-USP16 antibody (A05795-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP16 antigen affinity purified polyclonal antibody (Catalog # A05795-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP16 at approximately 110 kDa. The expected band size for USP16 is at 93 kDa.
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