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WB analysis of various sample lysates using GTX16439 USP16 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX16439 USP16 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX16439 USP16 antibody. Dilution : 1:1000 Loading : 25microg per lane

USP16 antibody

GTX16439
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetUSP16
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP16 antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10600
  • Target name
    USP16
  • Target description
    ubiquitin specific peptidase 16
  • Target synonyms
    deubiquitinating enzyme 16; ubiquitin carboxyl-terminal hydrolase 16; ubiquitin specific protease 16; ubiquitin thioesterase 16; ubiquitin thiolesterase 16; ubiquitin-processing protease UBP-M; ubiquitin-specific processing protease 16; UBPM; UBP-M
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y5T5
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 16
  • Scientific Description
    This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. Lei H et al., 2021 Jan 4, Nat Commun
    Read more

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Figure 1. Western blot analysis of USP16 using anti-USP16 antibody (A05795-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP16 antigen affinity purified polyclonal antibody (Catalog # A05795-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP16 at approximately 110 kDa. The expected band size for USP16 is at 93 kDa.
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