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WB analysis of extracts from mouse testis tissue using GTX02536 USP22 Antibody. The lane on the left was treated with blocking peptide.
WB analysis of extracts from mouse testis tissue using GTX02536 USP22 Antibody. The lane on the left was treated with blocking peptide.
WB analysis of extracts from mouse testis tissue using GTX02536 USP22 Antibody. The lane on the left was treated with blocking peptide.

USP22 antibody

GTX02536
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetUSP22
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP22 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID23326
  • Target name
    USP22
  • Target description
    ubiquitin specific peptidase 22
  • Target synonyms
    USP3L, ubiquitin carboxyl-terminal hydrolase 22, deubiquitinating enzyme 22, ubiquitin specific protease 22, ubiquitin thioesterase 22, ubiquitin thiolesterase 22, ubiquitin-specific processing protease 22
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UPT9
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 22
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP22 using anti-USP22 antibody (A03887-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human CACO-2 whole cell lysates, Lane 5: human MOLT-4 whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP22 antigen affinity purified polyclonal antibody (Catalog # A03887-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP22 at approximately 68 kDa. The expected band size for USP22 is at 60 kDa.
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