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WB analysis of various sample lysates using GTX55837 USP26 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55837 USP26 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX55837 USP26 antibody. Dilution : 1:1000 Loading : 25microg per lane

USP26 antibody

GTX55837
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetUSP26
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Overview

  • Supplier
    GeneTex
  • Product Name
    USP26 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID83844
  • Target name
    USP26
  • Target description
    ubiquitin specific peptidase 26
  • Target synonyms
    deubiquitinating enzyme 26; ubiquitin carboxyl-terminal hydrolase 26; ubiquitin specific protease 26; ubiquitin thioesterase 26; ubiquitin thiolesterase 26; ubiquitin-specific processing protease 26
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BXU7
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 26
  • Scientific Description
    This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases and is a deubiquitinating enzyme (DUB) with His and Cys domains. It is specifically expressed in testis tissue. Mutations in this gene have been associated with Sertoli cell-only syndrome and male infertility. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of USP26 using anti-USP26 antibody (A07732-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP26 antigen affinity purified polyclonal antibody (Catalog # A07732-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP26 at approximately 104 kDa. The expected band size for USP26 is at 104 kDa.
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