More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/USP5 Mouse Monoclonal Antibody [Clone ID: OTI2E3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY USP5 (Cat# RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5(Cat# TA501290). Positive lysates [LY418652] (100ug) and [LC418652] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY USP5 (Cat# RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5(Cat# TA501290). Positive lysates [LY418652] (100ug) and [LC418652] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY USP5 (Cat# RC202624, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-USP5(Cat# TA501290). Positive lysates [LY418652] (100ug) and [LC418652] (20ug) can be purchased separately from OriGene.

USP5 Mouse Monoclonal Antibody [Clone ID: OTI2E3]

CF501290
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetUSP5
Price on request
100 ug
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    OriGene
  • Product Name
    USP5 Mouse Monoclonal Antibody [Clone ID: OTI2E3]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E3
  • Gene ID8078
  • Target name
    USP5
  • Target description
    ubiquitin specific peptidase 5
  • Target synonyms
    ISOT, ubiquitin carboxyl-terminal hydrolase 5, deubiquitinating enzyme 5, isopeptidase T, testicular tissue protein Li 218, ubiquitin isopeptidase T, ubiquitin specific protease 5 (isopeptidase T), ubiquitin thioesterase 5, ubiquitin thiolesterase 5, ubiquitin-specific protease-5 (ubiquitin isopeptidase T), ubiquitin-specific-processing protease 5
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP45974
  • Protein Name
    Ubiquitin carboxyl-terminal hydrolase 5
  • Scientific Description
    Carrier-free (BSA/glycerol-free) USP5 mouse monoclonal antibody, clone OTI2E3 (formerly 2E3)
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

Download MSDS

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
USP5 AntibodyCAB4202
ReactivityHuman
TargetUSP5
  • SizePrice
Western blot All lanes: USP5 antibody at 10microg/ml Lane 1: Mouse brain tissue Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Lane 4: A375 whole cell lysate Lane 5: U251 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 96, 94 kDa Observed band size: 96 kDa
Product group Antibodies
Cusabio
USP5 AntibodyCSB-PA025742LA01HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetUSP5
  • SizePrice
Anti-USP5 Antibody
Product group Antibodies
Antibodies.com
Anti-USP5 AntibodyA14537
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity in cells in seminiferus ducts.
Product group Antibodies
Atlas Antibodies
Anti-USP5 AntibodyHPA006756
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetUSP5
  • SizePrice
Figure 1. Western blot analysis of USP5 using anti-USP5 antibody (A04550-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: huamn MCF-7 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP5 antigen affinity purified polyclonal antibody (Catalog # A04550-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP5 at approximately 96 kDa. The expected band size for USP5 is at 96 kDa.
Product group Antibodies
Boster Bio
Anti-USP5 Antibody Picoband(r)A04550-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetUSP5
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
USP5 AntibodyLS-C668076
ApplicationsWestern Blot
ReactivityHuman
TargetUSP5
  • SizePrice
Product group Antibodies
Biomatik
Usp5 Polyclonal AntibodyCAC07377
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
TargetUSP5
  • SizePrice
USP5 antibody [C1C3] detects USP5 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. USP5 antibody [C1C3] (GTX105407) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
GeneTex
USP5 antibody [C1C3]GTX105407
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetUSP5
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-USP5Y158093
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
Bioss
Ubiquitin Specific Peptidase 5 Recombinant Antbody Kit (KD-Validated)BSM-90506R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetUSP5
  • SizePrice