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ProductsBack/VAChT Antibody (OASE00241)

VAChT Antibody (OASE00241)

OASE00241
Aviva Systems Biology
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetSLC18A3
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    VAChT Antibody (OASE00241)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S6-38
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6572
  • Target name
    SLC18A3
  • Target description
    solute carrier family 18 member A3
  • Target synonyms
    CMS21, VACHT, vesicular acetylcholine transporter, solute carrier family 18 (vesicular acetylcholine transporter), member 3, solute carrier family 18 (vesicular acetylcholine), member 3, solute carrier family 18, member 3
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    VAChT is a member of the vesicular amine transporter (VMAT) family. The encoded transmembrane protein transports acetylcholine into secretory vesicle for release into the extracellular space. Acetylcholine (Ach) transport utilizes a proton gradient established by a vacuolar ATPase. This gene is located within the first intron of the choline acetyltransferase gene.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SLC18A3 using anti-SLC18A3 antibody (A04891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates, lane 2: HEPA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A3 antigen affinity purified polyclonal antibody (Catalog # A04891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A3 at approximately 55KD. The expected band size for SLC18A3 is at 57KD.
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