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ProductsBack/VAMP Associated Protein A Recombinant Antbody Kit (KD-Validated)

VAMP Associated Protein A Recombinant Antbody Kit (KD-Validated)

BSM-90513R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetVAPA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    VAMP Associated Protein A Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID9218
  • Target name
    VAPA
  • Target description
    VAMP associated protein A
  • Target synonyms
    33 kDa VAMP-associated protein; epididymis secretory sperm binding protein; hVAP-33; VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa; VAMP-A; VAP33; VAP-33; VAP-A; vesicle-associated membrane protein-associated protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0L0
  • Protein Name
    Vesicle-associated membrane protein-associated protein A
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VAPA using anti-VAPA antibody (A05329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPA antigen affinity purified polyclonal antibody (Catalog # A05329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPA at approximately 33 kDa. The expected band size for VAPA is at 28 kDa.
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