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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126671(VAPA Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126671(VAPA Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA126671(VAPA Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: x200)

VAPA Antibody

CSB-PA126671
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetVAPA
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Overview

  • Supplier
    Cusabio
  • Product Name
    VAPA Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9218
  • Target name
    VAPA
  • Target description
    VAMP associated protein A
  • Target synonyms
    33 kDa VAMP-associated protein; epididymis secretory sperm binding protein; hVAP-33; VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa; VAMP-A; VAP33; VAP-33; VAP-A; vesicle-associated membrane protein-associated protein A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P0L0
  • Protein Name
    Vesicle-associated membrane protein-associated protein A
  • Scientific Description
    The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VAPA using anti-VAPA antibody (A05329-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAPA antigen affinity purified polyclonal antibody (Catalog # A05329-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAPA at approximately 33 kDa. The expected band size for VAPA is at 28 kDa.
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