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Western Blot Positive WB detected in: Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue All lanes: VAMP7 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25, 31, 21 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue All lanes: VAMP7 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25, 31, 21 kDa Observed band size: 25 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse liver tissue, Mouse kidney tissue All lanes: VAMP7 antibody at 2microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25, 31, 21 kDa Observed band size: 25 kDa

VAMP7 Antibody

CSB-PA025785LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetVAMP7
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Overview

  • Supplier
    Cusabio
  • Product Name
    VAMP7 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6845
  • Target name
    VAMP7
  • Target description
    vesicle associated membrane protein 7
  • Target synonyms
    SYBL1; synaptobrevin-like 1; synaptobrevin-like protein 1; tetanus neurotoxin-insensitive VAMP; tetanus-insensitive VAMP; TIVAMP; TI-VAMP; VAMP-7; vesicle-associated membrane protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51809
  • Protein Name
    Vesicle-associated membrane protein 7
  • Scientific Description
    Involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. Required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. Required for calcium regulated lysosomal exocytosis. Involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. Required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. Required for focal exocytosis of late endocytic vesicles during phagosome formation.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SYBL1 using anti-SYBL1 antibody (PB10017). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYBL1 antigen affinity purified polyclonal antibody (Catalog # PB10017) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SYBL1 at approximately 25 kDa. The expected band size for SYBL1 is at 25 kDa.
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