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Vascular Endothelial Growth Factor C (VEGFC) Monoclonal Antibody

CAU33192
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetVegfc
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Overview

  • Supplier
    Biomatik
  • Product Name
    Vascular Endothelial Growth Factor C (VEGFC) Monoclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    C2
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID22341
  • Target name
    Vegfc
  • Target description
    vascular endothelial growth factor C
  • Target synonyms
    AW228853; flt4 ligand; flt4-L; vascular endothelial growth factor C; vascular endothelial growth factor-related protein; VEGF-; VEGF-C; VRP
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP97953
  • Protein Name
    Vascular endothelial growth factor C
  • Scientific Description
    The Vascular Endothelial Growth Factor C (VEGFC) Monoclonal Antibody (Species: Mouse) has been validated for the following applications: WB, IHC, ICC, IP.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Vegfc using anti-Vegfc antibody (A00623-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfc antigen affinity purified polyclonal antibody (Catalog # A00623-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfc at approximately 46 kDa. The expected band size for Vegfc is at 46 kDa.
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Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.
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References
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