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Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat small intestine; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with VEGF-C Polyclonal Antibody, Unconjugated (bs-1586R) at 1:400 overnight at 4°C, DAB staining.

VEGF-C Polyclonal Antibody

BS-1586R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVegfc
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Overview

  • Supplier
    Bioss
  • Product Name
    VEGF-C Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID22341
  • Target name
    Vegfc
  • Target description
    vascular endothelial growth factor C
  • Target synonyms
    VEGF-C, vascular endothelial growth factor C, VRP, flt4 ligand, flt4-L, vascular endothelial growth factor-related protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP97953
  • Protein Name
    Vascular endothelial growth factor C
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Ferrão JSP, Bonfim Neto AP, da Fonseca VU, et al. Vascular endothelial growth factor C treatment for mouse hind limb lymphatic revascularization. Vet Med Sci. 2019,5(2):249-259. doi: 10.1002/vms3.151
    Read this paper
  • Alunno A, Ibba-Manneschi L, Bistoni O, et al. Mobilization of lymphatic endothelial precursor cells and lymphatic neovascularization in primary Sjögren's syndrome. J Cell Mol Med. 2016,20(4):613-22. doi: 10.1111/jcmm.12793
    Read this paper
  • Shin YJ, Riew TR, Park JH, et al. Expression of vascular endothelial growth factor-C (VEGF-C) and its receptor (VEGFR-3) in the glial reaction elicited by human mesenchymal stem cell engraftment in the normal rat brain. J Histochem Cytochem. 2015,63(3):170-80. doi: 10.1369/0022155414564218
    Read this paper

Datasheet

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Figure 1. Western blot analysis of Vegfc using anti-Vegfc antibody (A00623-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vegfc antigen affinity purified polyclonal antibody (Catalog # A00623-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vegfc at approximately 46 kDa. The expected band size for Vegfc is at 46 kDa.
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