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VAV2 antibody

GTX131451
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
DownloadDatasheet
Product group Antibodies
TargetVAV2
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Overview

  • Supplier
    GeneTex
  • Product Name
    VAV2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:705. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.96 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7410
  • Target name
    VAV2
  • Target description
    vav guanine nucleotide exchange factor 2
  • Target synonyms
    guanine nucleotide exchange factor VAV2; vav 2 oncogene; VAV-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP52735
  • Protein Name
    Guanine nucleotide exchange factor VAV2
  • Scientific Description
    VAV2 is the second member of the VAV guanine nucleotide exchange factor family of oncogenes. Unlike VAV1, which is expressed exclusively in hematopoietic cells, VAV2 transcripts were found in most tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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HEK293 cells were transfected with either emply expression vector (pSR ) or pSRa-Vav2 driving expression of human Vav2. Indicated antibodies (SHD or DPH) were used to immunoprecipitate Vav2 from 300ug protein lysates. Immunoprecipitated activity was identified by immunoblot of complexes using either DPH (upper gel) or SHD (lower gel) antisera at (1:10000). Immunoprecipitation of Endogenous/Exogenous Vav2 from HEK 283 (75 u g lysate per lane). Top gel - i.p. Ab: DPH or SHD preimmune serum (2.5 l/300 g lysate). DPH or SHD alpha Vav2 antiserum (2.5 l/300 g lysate). Western Blot Ab: DPH anti Vav2 1:10000. Bottom Gel - i.p. Ab DPH or SHD preimmune sera (2.5 l/300 g lysate). DPH or SHD anti Vav2 antisera (2.5 l/300 g lysate). Western blot Ab - SHD anti Vav2 antibody 1:10000.
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