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ProductsBack/VAV3 (phospho Tyr173) antibody
WB (peptide competition) analysis of Cos7 cells over-expressing murine Vav3 were serum starved and left treated (Lane 1) or treated (Lane 2-5) with EGF using GTX24764 VAV3 (phospho Tyr173) antibody prior incubated with the non-phospho immunogen (Lane 3), a generic (pY) peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of Cos7 cells over-expressing murine Vav3 were serum starved and left treated (Lane 1) or treated (Lane 2-5) with EGF using GTX24764 VAV3 (phospho Tyr173) antibody prior incubated with the non-phospho immunogen (Lane 3), a generic (pY) peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.
WB (peptide competition) analysis of Cos7 cells over-expressing murine Vav3 were serum starved and left treated (Lane 1) or treated (Lane 2-5) with EGF using GTX24764 VAV3 (phospho Tyr173) antibody prior incubated with the non-phospho immunogen (Lane 3), a generic (pY) peptide (Lane 4), or, the phosphopeptide immunogen (Lane 5) control. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

VAV3 (phospho Tyr173) antibody

GTX24764
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetVAV3
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Overview

  • Supplier
    GeneTex
  • Product Name
    VAV3 (phospho Tyr173) antibody - Orthogonal Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-1 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10451
  • Target name
    VAV3
  • Target description
    vav guanine nucleotide exchange factor 3
  • Target synonyms
    guanine nucleotide exchange factor VAV3, VAV-3, vav 3 guanine nucleotide exchange factor, vav 3 oncogene
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKW4
  • Protein Name
    Guanine nucleotide exchange factor VAV3
  • Scientific Description
    This gene is a member of the VAV gene family. The VAV proteins are guanine nucleotide exchange factors (GEFs) for Rho family GTPases that activate pathways leading to actin cytoskeletal rearrangements and transcriptional alterations. This gene product acts as a GEF preferentially for RhoG, RhoA, and to a lesser extent, RAC1, and it associates maximally with the nucleotide-free states of these GTPases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VAV3 using anti-VAV3 antibody (A03204-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAV3 antigen affinity purified polyclonal antibody (Catalog # A03204-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VAV3 at approximately 98 kDa. The expected band size for VAV3 is at 98 kDa.
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