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Western Blot Positive WB detected in: K562 whole cell lysate,A549 whole cell lysate,293 whole cell lysate,U251 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate All lanes: VDAC3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: K562 whole cell lysate,A549 whole cell lysate,293 whole cell lysate,U251 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate All lanes: VDAC3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: K562 whole cell lysate,A549 whole cell lysate,293 whole cell lysate,U251 whole cell lysate,HL-60 whole cell lysate,Hela whole cell lysate All lanes: VDAC3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 32 kDa

VDAC3 Antibody

CSB-PA896484LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetVDAC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    VDAC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7419
  • Target name
    VDAC3
  • Target description
    voltage dependent anion channel 3
  • Target synonyms
    HD-VDAC3, VDAC-3, non-selective voltage-gated ion channel VDAC3, outer mitochondrial membrane protein porin 3, voltage-dependent anion-selective channel protein 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y277
  • Protein Name
    Non-selective voltage-gated ion channel VDAC3
  • Scientific Description
    Forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VDAC3 using anti-VDAC3 antibody (A04802-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat kidney tissue lysates, Lane 5: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VDAC3 antigen affinity purified polyclonal antibody (Catalog # A04802-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VDAC3 at approximately 31KD. The expected band size for VDAC3 is at 31KD.
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