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WB analysis of extracellular domain of human FLT1 using GTX83314 VEGF Receptor 1 antibody [3D10].
WB analysis of extracellular domain of human FLT1 using GTX83314 VEGF Receptor 1 antibody [3D10].
WB analysis of extracellular domain of human FLT1 using GTX83314 VEGF Receptor 1 antibody [3D10].

VEGF Receptor 1 antibody [3D10]

GTX83314
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetFLT1
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Overview

  • Supplier
    GeneTex
  • Product Name
    VEGF Receptor 1 antibody [3D10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D10
  • Conjugate
    Unconjugated
  • Gene ID2321
  • Target name
    FLT1
  • Target description
    fms related receptor tyrosine kinase 1
  • Target synonyms
    FLT; FLT-1; fms related tyrosine kinase 1; fms-like tyrosine kinase 1; fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor); tyrosine-protein kinase FRT; tyrosine-protein kinase receptor FLT; vascular endothelial growth factor receptor 1; vascular permeability factor receptor; VEGFR1; VEGFR-1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17948
  • Protein Name
    Vascular endothelial growth factor receptor 1
  • Scientific Description
    This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 150KD. The expected band size for VEGF Receptor 1 is at 150KD.
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