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ProductsBack/VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: 3D10]
Western blot analysis using FLT1 mouse mAb against extracellular domain of human FLT1.
Western blot analysis using FLT1 mouse mAb against extracellular domain of human FLT1.
Western blot analysis using FLT1 mouse mAb against extracellular domain of human FLT1.

VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: 3D10]

AM06366SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetFLT1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    VEGF Receptor 1 (FLT1) Mouse Monoclonal Antibody [Clone ID: 3D10]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D10
  • Gene ID2321
  • Target name
    FLT1
  • Target description
    fms related receptor tyrosine kinase 1
  • Target synonyms
    FLT, FLT-1, VEGFR-1, VEGFR1, vascular endothelial growth factor receptor 1, fms related tyrosine kinase 1, fms-like tyrosine kinase 1, fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor receptor), tyrosine-protein kinase FRT, tyrosine-protein kinase receptor FLT, vascular permeability factor receptor
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP17948
  • Protein Name
    Vascular endothelial growth factor receptor 1
  • Scientific Description
    VEGF Receptor 1 (FLT1) mouse monoclonal antibody, clone 3D10, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of VEGF Receptor 1 using anti-VEGF Receptor 1 antibody (A00534-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat kidney tissue lysate, Lane 2: mouse liver tissue lysate, Lane 3: mouse HEPA1-6 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VEGF Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A00534-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VEGF Receptor 1 at approximately 150KD. The expected band size for VEGF Receptor 1 is at 150KD.
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