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VEGF Receptor 2 antibody [7A23]

GTX53093
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityMouse
TargetKdr
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Overview

  • Supplier
    GeneTex
  • Product Name
    VEGF Receptor 2 antibody [7A23]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:200 - 1:800. IHC-Fr: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7A23
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID16542
  • Target name
    Kdr
  • Target description
    kinase insert domain protein receptor
  • Target synonyms
    6130401C07, Flk-1, Flk1, Krd-1, Ly73, VEGFR-2, VEGFR2, orv, sVEGFR-2, vascular endothelial growth factor receptor 2, VEGF receptor-2, fetal liver kinase 1, kinase NYK, protein-tyrosine kinase receptor flk-1, soluble vascular endothelial growth factor receptor 2, vascular endothelial growth factor receptor-3
  • Host
    Rat
  • Isotype
    IgG2
  • Reactivity
    Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VEGF Receptor 2 using anti-VEGF Receptor 2 antibody (A00901-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Marker Lane 2: mouse heart tissue lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse spleen tissue lysates, Lane 5: mouse kidney tissue lysates. Lane 6: mouse brain tissue lysates, Lane 5: mouse lung tissue lysates. Lane 7: mouse HEPA1-6 whole cell lysates, Lane 8: mouse NIH3T3 whole cell lysates. The membrane was incubated with rabbit anti-VEGF Receptor 2 antigen affinity purified polyclonal antibody (Catalog # A00901-3) at 1.0 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
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