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MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

VEGFR2 Polyclonal Antibody

BS-10412R
Bioss
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKDR
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Overview

  • Supplier
    Bioss
  • Product Name
    VEGFR2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3791
  • Target name
    KDR
  • Target description
    kinase insert domain receptor
  • Target synonyms
    CD309, FLK1, VEGFR, VEGFR2, vascular endothelial growth factor receptor 2, fetal liver kinase-1, kinase insert domain receptor (a type III receptor tyrosine kinase), protein-tyrosine kinase receptor Flk-1, soluble VEGFR2, tyrosine kinase growth factor receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP35968
  • Protein Name
    Vascular endothelial growth factor receptor 2
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Ding J et al., 2020 Jul 1, Int J Biol Macromol
    Read this paper
  • C3a receptor antagonist therapy is protective with or without thrombolysis in murine thromboembolic stroke. Ahmad S et al., 2020 Jun, Br J Pharmacol
    Read this paper
  • Silencing of RBP-JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Luo Z et al., 2020 Jan, Mol Med Rep
    Read this paper
  • Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension. Bhagwani AR et al., 2019, PLoS One
    Read this paper
  • Flow-enhanced vascularization and maturation of kidney organoids in vitro. Homan KA et al., 2019 Mar, Nat Methods
    Read this paper
  • Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model. Xiao D et al., 2017 Oct 17, Oncotarget
    Read this paper
  • Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model. Wang L et al., 2017 Apr, Peptides
    Read this paper

Datasheet

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