MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
MCF7 cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with VEGFR2 Primary Antibody （bs-10412R） for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
VEGFR2 Polyclonal Antibody
BS-10412R
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetKDR
Overview
- SupplierBioss
- Product NameVEGFR2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID3791
- Target nameKDR
- Target descriptionkinase insert domain receptor
- Target synonymsCD309, FLK1, VEGFR, VEGFR2, vascular endothelial growth factor receptor 2, fetal liver kinase-1, kinase insert domain receptor (a type III receptor tyrosine kinase), protein-tyrosine kinase receptor Flk-1, soluble VEGFR2, tyrosine kinase growth factor receptor
- HostRabbit
- IsotypeIgG
- Protein IDP35968
- Protein NameVascular endothelial growth factor receptor 2
- Storage Instruction-20°C
- UNSPSC12352203
References
- Ding J, Jia W, Cui Y, et al. Anti-angiogenic effect of a chemically sulfated polysaccharide from Phellinus ribis by inhibiting VEGF/VEGFR pathway. Int J Biol Macromol. 2020,154:72-81. doi: 10.1016/j.ijbiomac.2020.03.068Read this paper
- Ahmad S, Pandya C, Kindelin A, et al. C3a receptor antagonist therapy is protective with or without thrombolysis in murine thromboembolic stroke. Br J Pharmacol. 2020,177(11):2466-2477. doi: 10.1111/bph.14989Read this paper
- Luo Z, Dai Y, Chen M, et al. Silencing of RBP‑JK promotes the differentiation of bone marrow mesenchymal stem cells into vascular endothelial cells. Mol Med Rep. 2020,21(1):69-76. doi: 10.3892/mmr.2019.10803Read this paper
- Bhagwani AR, Hultman S, Farkas D, et al. Endothelial cells are a source of Nestin expression in Pulmonary Arterial Hypertension. PLoS One. 2019,14(3):e0213890. doi: 10.1371/journal.pone.0213890Read this paper
- Xiao D, Wang Q, Yan H, et al. Adipose-derived stem cells-seeded bladder acellular matrix graft-silk fibroin enhances bladder reconstruction in a rat model. Oncotarget. 2017,8(49):86471-86487. doi: 10.18632/oncotarget.21211Read this paper
- Wang L, Chen Q, Ke D, et al. Ghrelin inhibits atherosclerotic plaque angiogenesis and promotes plaque stability in a rabbit atherosclerotic model. Peptides. 2017,90:17-26. doi: 10.1016/j.peptides.2017.01.013Read this paper
Datasheet
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