VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20220902_WB_B_22092119_541.webp "Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_T-44732_20221014_WB_M_brain_22101718_856.webp "Boiled and unboiled mouse tissue extract (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1616] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221007_IHC-P_M_1_22110201_204.webp "VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse eye. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Red: beta Tubulin 3/ Tuj1, a Cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221007_IHC-P_M_22110201_109.webp "VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Red: NF-H, a Cytoplasm marker, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_T-44732_20221202_IHC-Fr_M_22121123_949.webp "VGAT antibody [HL1616] detects VGAT protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100.")
![VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20221209_ICC_IF_R_22122018_363.webp "VGAT antibody [HL1616] detects VGAT protein at nucleus and cytoskeleton by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:250. Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
![VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_44788_20230526_IHC-Fr_M_23053001_291.webp "VGAT antibody [HL1616] detects VGAT protein at cell membrane by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Blue: Fluoroshield with DAPI (GTX30920).")
![Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637107/GTX637107_45278_20240105_WB_B_24110700_742.webp "Non-transfected (–) and transfected (+) unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with VGAT antibody [HL1615] (GTX637107) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
VGAT antibody [HL1616] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1616] (GTX637107) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
VGAT antibody [HL1616]
GTX637107
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSLC32A1
Overview
- SupplierGeneTex
- Product NameVGAT antibody [HL1616]
- Delivery Days Customer9
- Application Supplier NoteIHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1616
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID140679
- Target nameSLC32A1
- Target descriptionsolute carrier family 32 member 1
- Target synonymsDEE114, GEFSP12, VGAT, VIAAT, VIAAT GEFSP12, vesicular inhibitory amino acid transporter, GABA and glycine transporter, hVIAAT, solute carrier family 32 (GABA vesicular transporter), member 1, vesicular GABA transporter
- HostRabbit
- IsotypeIgG
- Protein IDQ9H598
- Protein NameVesicular inhibitory amino acid transporter
- Scientific DescriptionThe protein encoded by this gene is an integral membrane protein involved in gamma-aminobutyric acid (GABA) and glycine uptake into synaptic vesicles. The encoded protein is a member of amino acid/polyamine transporter family II. [provided by RefSeq, Jul 2008]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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![VGAT antibody [N1N2], N-term detects VGAT protein expression by immunofluorescent analysis. Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VGAT protein stained by VGAT antibody [N1N2], N-term (GTX101908) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101908/GTX101908_40275_20161004_IFA_w_23060100_323.webp)
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![VGAT antibody [HL1615] detects VGAT protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse cerebellum. VGAT stained by VGAT antibody [HL1615] (GTX637106) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637106/GTX637106_T-44732_20220805_IHC-P_M_22080923_282.webp)
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