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ProductsBack/Vinculin antibody [N3C1], Internal
Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000
Vinculin antibody [N3C1], Internal detects VCL protein by Western blot analysis. A. 30 μg PC-12 whole cell lysate/extract B. 30 μg Rat2 whole cell lysate/extract 5 % SDS-PAGE Vinculin antibody [N3C1], Internal (GTX109749) dilution: 1:1000

Vinculin antibody [N3C1], Internal

GTX109749
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat
TargetVCL
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Overview

  • Supplier
    GeneTex
  • Product Name
    Vinculin antibody [N3C1], Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.11 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7414
  • Target name
    VCL
  • Target description
    vinculin
  • Target synonyms
    CMD1W, CMH15, HEL114, MV, MVCL, VINC, vinculin, epididymis luminal protein 114, epididymis secretory sperm binding protein, meta-vinculin, metavinculin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18206
  • Protein Name
    Vinculin
  • Scientific Description
    Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Lee YZ, Hsu HY, Yang CW, et al. The Synergistic Inhibition of Coronavirus Replication and Induced Cytokine Production by Ciclesonide and the Tylophorine-Based Compound Dbq33b. Pharmaceutics. 2022,14(7). doi: 10.3390/pharmaceutics14071511
    Read this paper
  • Yang CW, Lee YZ, Hsu HY, et al. Tyrphostin AG1024 Suppresses Coronaviral Replication by Downregulating JAK1 via an IR/IGF-1R Independent Proteolysis Mediated by Ndfip1/2_NEDD4-like E3 Ligase Itch. Pharmaceuticals (Basel). 2022,15(2). doi: 10.3390/ph15020241
    Read this paper
  • Novak JS, Spathis R, Dang UJ, et al. Interrogation of Dystrophin and Dystroglycan Complex Protein Turnover After Exon Skipping Therapy. J Neuromuscul Dis. 2021,8(s2):S383-S402. doi: 10.3233/JND-210696
    Read this paper
  • Cunningham S, McCauley S, Vairamani K, et al. FLASH Proton Pencil Beam Scanning Irradiation Minimizes Radiation-Induced Leg Contracture and Skin Toxicity in Mice. Cancers (Basel). 2021,13(5). doi: 10.3390/cancers13051012
    Read this paper
  • Macchi C, Greco MF, Botta M, et al. Leptin, Resistin, and Proprotein Convertase Subtilisin/Kexin Type 9: The Role of STAT3. Am J Pathol. 2020,190(11):2226-2236. doi: 10.1016/j.ajpath.2020.07.016
    Read this paper
  • Huang HY, Chou HC, Law CH, et al. Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis. J Cell Mol Med. 2020,24(17):9737-9751. doi: 10.1111/jcmm.15535
    Read this paper
  • Yang CW, Hsu HY, Chang HY, et al. Natural cardenolides suppress coronaviral replication by downregulating JAK1 via a Na(+)/K(+)-ATPase independent proteolysis. Biochem Pharmacol. 2020,180:114122. doi: 10.1016/j.bcp.2020.114122
    Read this paper
  • Chou CW, Lin CH, Hsiao TH, et al. Therapeutic effects of statins against lung adenocarcinoma via p53 mutant-mediated apoptosis. Sci Rep. 2019,9(1):20403. doi: 10.1038/s41598-019-56532-6
    Read this paper
  • Lee YZ, Guo HC, Zhao GH, et al. Tylophorine-based compounds are therapeutic in rheumatoid arthritis by targeting the caprin-1 ribonucleoprotein complex and inhibiting expression of associated c-Myc and HIF-1α. Pharmacol Res. 2020,152:104581. doi: 10.1016/j.phrs.2019.104581
    Read this paper
  • Panneerselvam L, Raghunath A, Ravi MS, et al. Ferulic acid attenuates arsenic-induced cardiotoxicity in rats. Biotechnol Appl Biochem. 2020,67(2):186-195. doi: 10.1002/bab.1830
    Read this paper

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Figure 1. Western blot analysis of VCL using anti-VCL antibody (A01207-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HT1080 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human SK-OV-3 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human SGC-7901 whole cell lysates, Lane 9: rat heart tissue lysates, Lane 10: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCL antigen affinity purified polyclonal antibody (Catalog # A01207-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCL at approximately 124KD. The expected band size for VCL is at 124KD.
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