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VIPR1 Antibody (aa305-334, APC)

LS-C218421
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetVIPR1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    VIPR1 Antibody (aa305-334, APC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    APC (Allophycocyanin)
  • Estimated Purity
    ...
  • Gene ID7433
  • Target name
    VIPR1
  • Target description
    vasoactive intestinal peptide receptor 1
  • Target synonyms
    HVR1; II; PACAP type II receptor; PACAP-R2; PACAP-R-2; pituitary adenylate cyclase activating polypeptide receptor, type II; RDC1; type 1 vasoactive intestinal peptide receptor; V1RG; VAPC1; vasoactive intestinal polypeptide receptor 1; VIP and PACAP receptor 1; VIP receptor, type I; VIPR; VIP-R-1; VIRG; VPAC1; VPAC1 receptor; VPAC1R; VPCAP1R
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti VIP Receptor 1/VAPC1 Polyclonal Antibody, Unconjugated (bs-2982R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD
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Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 cell lysates, Lane 2: human PANC-1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A04345) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.
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