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Western Blot analysis of HepG2 cells using VPAC1 Polyclonal Antibody.
Western Blot analysis of HepG2 cells using VPAC1 Polyclonal Antibody.
Western Blot analysis of HepG2 cells using VPAC1 Polyclonal Antibody.

VIPR1 Antibody

CSB-PA006153
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetVIPR1
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Overview

  • Supplier
    Cusabio
  • Product Name
    VIPR1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7433
  • Target name
    VIPR1
  • Target description
    vasoactive intestinal peptide receptor 1
  • Target synonyms
    HVR1; II; PACAP type II receptor; PACAP-R2; PACAP-R-2; pituitary adenylate cyclase activating polypeptide receptor, type II; RDC1; type 1 vasoactive intestinal peptide receptor; V1RG; VAPC1; vasoactive intestinal polypeptide receptor 1; VIP and PACAP receptor 1; VIP receptor, type I; VIPR; VIP-R-1; VIRG; VPAC1; VPAC1 receptor; VPAC1R; VPCAP1R
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32241
  • Protein Name
    Vasoactive intestinal polypeptide receptor 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Lane 1: rat brain lysates Lane 2: human colon carcinoma lysates probed with Anti VIP Receptor 1/VAPC1 Polyclonal Antibody, Unconjugated (bs-2982R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 47kD. Observed band size: 47kD
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Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 cell lysates, Lane 2: human PANC-1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A04345) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.
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