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VLDL Receptor Polyclonal Antibody, PE Conjugated

BS-4228R-PE
Bioss
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityCanine, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetVLDLR
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Overview

  • Supplier
    Bioss
  • Product Name
    VLDL Receptor Polyclonal Antibody, PE Conjugated
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    RPE
  • Gene ID7436
  • Target name
    VLDLR
  • Target description
    very low density lipoprotein receptor
  • Target synonyms
    CAMRQ1, CARMQ1, CHRMQ1, VLDL-R, VLDLRCH, very low-density lipoprotein receptor, VLDL receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP98155
  • Protein Name
    Very low-density lipoprotein receptor
  • Reactivity
    Canine, Equine, Human, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VLDL Receptor/VLDLR using anti-VLDL Receptor/VLDLR antibody (A02195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VLDL Receptor/VLDLR antigen affinity purified polyclonal antibody (Catalog # A02195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VLDL Receptor/VLDLR at approximately 100 kDa. The expected band size for VLDL Receptor/VLDLR is at 100 kDa.
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