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Immunohistochemistry of paraffin-embedded Human liver cancer tissue using VLDLR Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using VLDLR Polyclonal Antibody at dilution 1:40
Immunohistochemistry of paraffin-embedded Human liver cancer tissue using VLDLR Polyclonal Antibody at dilution 1:40

VLDLR Polyclonal Antibody

E-AB-17551
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    VLDLR Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. This gene encodes a lipoprotein receptor that is a member of the LDLR family and plays important roles in VLDL-triglyceride metabolism and the reelin signaling pathway. Mutations in this gene cause VLDLR-associated cerebellar hypoplasia. Alternative splicing generates multiple transcript variants encoding distinct isoforms for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of VLDL Receptor/VLDLR using anti-VLDL Receptor/VLDLR antibody (A02195-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VLDL Receptor/VLDLR antigen affinity purified polyclonal antibody (Catalog # A02195-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VLDL Receptor/VLDLR at approximately 100 kDa. The expected band size for VLDL Receptor/VLDLR is at 100 kDa.
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IHC image of CSB-PA025865LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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HEK 239T cells probed with VLDL Receptor Polyclonal Antibody, Unconjugated (bs-4228R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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