VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108058/GTX108058_41703_20180907_WB_shRNA_watermark_w_23060120_976.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX108058/GTX108058_39721_IHC_w_23060120_623.webp "VPS35 antibody [C3], C-term detects VPS35 protein at cytoplasm and membrane on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. VPS35 antibody [C3], C-term (GTX108058) dilution: 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX108058/GTX108058_43950_20200522_WB_w_23060120_329.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [C3], C-term (GTX108058) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
VPS35 antibody [C3], C-term detects VPS35 protein at endosome by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS35 stained by VPS35 antibody [C3], C-term (GTX108058) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
VPS35 antibody [C3], C-term
GTX108058
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetVPS35
Overview
- SupplierGeneTex
- Product NameVPS35 antibody [C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.66 mg/ml
- ConjugateUnconjugated
- Gene ID55737
- Target nameVPS35
- Target descriptionVPS35 retromer complex component
- Target synonymsMEM3, PARK17, vacuolar protein sorting-associated protein 35, hVPS35, maternal-embryonic 3, vacuolar protein sorting 35 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ96QK1
- Protein NameVacuolar protein sorting-associated protein 35
- Scientific DescriptionThis gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Rahman AA, Soto-Avellaneda A, Yong Jin H, et al. Enhanced Hyaluronan Signaling and Autophagy Dysfunction by VPS35 D620N. Neuroscience. 2020,441:33-45. doi: 10.1016/j.neuroscience.2020.06.009Read this paper
- Beilina A, Bonet-Ponce L, Kumaran R, et al. The Parkinson's Disease Protein LRRK2 Interacts with the GARP Complex to Promote Retrograde Transport to the trans-Golgi Network. Cell Rep. 2020,31(5):107614. doi: 10.1016/j.celrep.2020.107614Read this paper
- Lenoir M, Ustunel C, Rajesh S, et al. Phosphorylation of conserved phosphoinositide binding pocket regulates sorting nexin membrane targeting. Nat Commun. 2018,9(1):993. doi: 10.1038/s41467-018-03370-1Read this paper
- Succoio M, Comegna M, D'Ambrosio C, et al. Proteomic analysis reveals novel common genes modulated in both replicative and stress-induced senescence. J Proteomics. 2015,128:18-29. doi: 10.1016/j.jprot.2015.07.010Read this paper
- Miura E, Hasegawa T, Konno M, et al. VPS35 dysfunction impairs lysosomal degradation of α-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease. Neurobiol Dis. 2014,71:1-13. doi: 10.1016/j.nbd.2014.07.014Read this paper
Datasheet
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