Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20221216_WB_Z_22122018_613.webp "Whole zebrafish extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.")
![VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20221228_IHC-P_R_22122901_411.webp "VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20221228_IHC-P_multiple_M_22122901_216.webp "VPS35 antibody [HL1017] detects VPS35 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissue. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_T-44102_20210730_IHC-P_w_23061202_587.webp "VPS35 antibody [HL1017] detects VPS35 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. VPS35 stained by VPS35 antibody [HL1017] (GTX635821) diluted at 1:50. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20210115_WB_w_23061202_441.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20201204_WB_w_23061202_994.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20210115_WB_M_R_w_23061202_518.webp "Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX635821/GTX635821_44151_20210101_WB_shRNA_watermark_w_23061202_860.webp "Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with VPS35 antibody [HL1017] (GTX635821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
VPS35 antibody [HL1017]
GTX635821
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
TargetVPS35
Overview
- SupplierGeneTex
- Product NameVPS35 antibody [HL1017]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1017
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID55737
- Target nameVPS35
- Target descriptionVPS35 retromer complex component
- Target synonymsMEM3, PARK17, vacuolar protein sorting-associated protein 35, hVPS35, maternal-embryonic 3, vacuolar protein sorting 35 homolog
- HostRabbit
- IsotypeIgG
- Protein IDQ96QK1
- Protein NameVacuolar protein sorting-associated protein 35
- Scientific DescriptionThis gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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