More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/VRK1 antibody [1F6]
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of HeLa cells using GTX79470 VRK1 antibody [1F6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 1:100 dilution in 0.1% BSA and incubated for 3 hours at room temperature

VRK1 antibody [1F6]

GTX79470
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetVRK1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    VRK1 antibody [1F6]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. ICC/IF: 1:100. IHC-P: 1:100 - 1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1F6
  • Conjugate
    Unconjugated
  • Gene ID7443
  • Target name
    VRK1
  • Target description
    VRK serine/threonine kinase 1
  • Target synonyms
    HMNR10, PCH1, PCH1A, serine/threonine-protein kinase VRK1, vaccinia related kinase 1, vaccinia virus B1R-related kinase 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ99986
  • Protein Name
    Serine/threonine-protein kinase VRK1
  • Scientific Description
    This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
VRK1 AntibodyCAB7745
ReactivityHuman
TargetVRK1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-VRK1 Antibody144-07745
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetVRK1
  • SizePrice
Anti-VRK1 Antibody
Product group Antibodies
Antibodies.com
Anti-VRK1 AntibodyA44505
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
VRK1 AntibodyLS-C831877
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetVRK1
  • SizePrice
Western blot analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1, using Anti-VRK1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Product group Antibodies
Atlas Antibodies
Anti-VRK1 AntibodyHPA000660
ApplicationsWestern Blot
ReactivityHuman
TargetVRK1
  • SizePrice
Western blot All lanes: DCXR antibody at 4.74ug/ml Lane 1: MCF-7 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: A549 whole cell lysate Lane 4: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
Product group Antibodies
Cusabio
VRK1 AntibodyCSB-PA857466DSR1HU
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetVRK1
  • SizePrice
Product group Antibodies
Bioss
VRK1 Polyclonal AntibodyBS-7706R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Equine, Human, Mouse, Porcine, Rat, Sheep
TargetVRK1
  • SizePrice
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE GTX107899 diluted at 1:1000
Product group Antibodies
GeneTex
VRK1 antibodyGTX107899
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetVRK1
  • SizePrice
IHC-P analysis of mouse lung tissue using GTX33581 VRK1 antibody. Dilution : 1:100
Product group Antibodies
GeneTex
VRK1 antibodyGTX33581
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetVRK1
  • SizePrice
Figure 1. Western blot analysis of VRK1 using anti-VRK1 antibody (PB9907). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: JURKAT whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VRK1 antigen affinity purified polyclonal antibody (Catalog # PB9907) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VRK1 at approximately 55 kDa. The expected band size for VRK1 is at 45 kDa.
Product group Antibodies
Boster Bio
Anti-VRK1 Antibody Picoband(r)PB9907-CARRIER-FREE
ApplicationsWestern Blot
ReactivityHuman, Rat
TargetVRK1
  • SizePrice