ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : Methanol
![ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : 4% PFA](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_ICC-IF_2_w_23053121_314.webp "ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : 4% PFA")
![ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : Methanol](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_ICC-IF_3_w_23053121_347.webp "ICC/IF analysis of U2OS cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : Methanol")
![ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Lower panel : Metaphase cells Dilution : 1:100 Fixation : 4% PFA](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_ICC-IF_w_23053121_895.webp "ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Lower panel : Metaphase cells Dilution : 1:100 Fixation : 4% PFA")
![WB analysis of HeLa cell lysate using GTX00703 VRK1 antibody [5D1]. Samples : Lane 1 : HeLa cells transfected Luciferase RNAi (control) Lane 2 : HeLa cells transfected VRK1-1 RNAi Lane 3 : HeLa cells transfected VRK1-2 RNAi Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_WB_1_w_23053121_705.webp "WB analysis of HeLa cell lysate using GTX00703 VRK1 antibody [5D1]. Samples : Lane 1 : HeLa cells transfected Luciferase RNAi (control) Lane 2 : HeLa cells transfected VRK1-1 RNAi Lane 3 : HeLa cells transfected VRK1-2 RNAi Dilution : 1:500")
![WB analysis of TIG-1 cell lysate using GTX00703 VRK1 antibody [5D1]. Samples : Lane 1 : TIG-1 cells transfected Luciferase RNAi (control) Lane 2 : TIG-1 cells transfected VRK1-1 RNAi Lane 3 : TIG-1 cells transfected VRK1-2 RNAi Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_WB_2_w_23053121_967.webp "WB analysis of TIG-1 cell lysate using GTX00703 VRK1 antibody [5D1]. Samples : Lane 1 : TIG-1 cells transfected Luciferase RNAi (control) Lane 2 : TIG-1 cells transfected VRK1-1 RNAi Lane 3 : TIG-1 cells transfected VRK1-2 RNAi Dilution : 1:500")
![WB analysis of various samples using GTX00703 VRK1 antibody [5D1]. Lane 1-3 : HeLa whole cell lysate Lane 4-6 : U2OS whole cell lysate Dilution : Lane 1, 4 : 1:100 Lane 2, 5 : 1:500 Lane 3, 6 : 1:1000 Cell amount : 5 x10? cells](https://www.genetex.com/upload/website/prouct_img/normal/GTX00703/GTX00703_20191104_WB_w_23053121_577.webp "WB analysis of various samples using GTX00703 VRK1 antibody [5D1]. Lane 1-3 : HeLa whole cell lysate Lane 4-6 : U2OS whole cell lysate Dilution : Lane 1, 4 : 1:100 Lane 2, 5 : 1:500 Lane 3, 6 : 1:1000 Cell amount : 5 x10? cells")
ICC/IF analysis of HeLa cells using GTX00703 VRK1 antibody [5D1]. Upper panel : Interphase cells Middle and lower panel : Metaphase cells Dilution : 1:100 Fixation : Methanol
VRK1 antibody [5D1] - KO/KD, Orthogonal Validated
GTX00703
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetVRK1
Overview
- SupplierGeneTex
- Product NameVRK1 antibody [5D1] - KO/KD, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID5D1
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID7443
- Target nameVRK1
- Target descriptionVRK serine/threonine kinase 1
- Target synonymsPCH1; PCH1A; serine/threonine-protein kinase VRK1; vaccinia related kinase 1; vaccinia virus B1R-related kinase 1
- HostMouse
- IsotypeIgG1
- Scientific DescriptionThis gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
- ReactivityHuman
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203