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WAC Antibody

LS-C201071
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetWAC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WAC Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    WAC Antibody can be used for detection of WAC by Western blot at 1 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 71 kDa Observed: 75 kDa. ELISA, IF (5 µg/ml), IHC (2.5 µg/ml), WB (1 µg/ml) WAC Antibody can be used for detection of WAC by Western blot at 1 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot: Predicted: 71 kDa Observed: 75 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51322
  • Target name
    WAC
  • Target description
    WW domain containing adaptor with coiled-coil
  • Target synonyms
    BM-016, DESSH, PRO1741, Wwp4, WW domain-containing adapter protein with coiled-coil
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of WAC using anti-WAC antibody (A04941-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human Raji whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human U20S whole cell lysates, Lane 6: human SK-OV-3 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse thymus tissue lysates, Lane 10: mouse SP2/0 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WAC antigen affinity purified polyclonal antibody (Catalog # A04941-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WAC at approximately 85KD. The expected band size for WAC is at 85KD.
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