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ProductsBack/WC1 / ATP7B Antibody (aa3-21, clone S62-29, APC)

WC1 / ATP7B Antibody (aa3-21, clone S62-29, APC)

LS-C228600
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetATP7B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    WC1 / ATP7B Antibody (aa3-21, clone S62-29, APC)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P, IP, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    S62-29
  • Concentration
    1 mg/ml
  • Conjugate
    APC (Allophycocyanin)
  • Estimated Purity
    ...
  • Gene ID540
  • Target name
    ATP7B
  • Target description
    ATPase copper transporting beta
  • Target synonyms
    ATPase, Cu(2+)- transporting, beta polypeptide; ATPase, Cu++ transporting, beta polypeptide; copper pump 2; copper-transporting ATPase 2; copper-transporting protein ATP7B; PWD; WC1; WD; Wilson disease-associated protein; WND
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATP7B using anti-ATP7B antibody (A00686-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP7B antigen affinity purified polyclonal antibody (Catalog # A00686-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP7B at approximately 157 kDa. The expected band size for ATP7B is at 157 kDa.
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