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Western blot All lanes: Methylosome protein 50 antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 30 kDa Observed band size: 37 kDa
Western blot All lanes: Methylosome protein 50 antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 30 kDa Observed band size: 37 kDa
Western blot All lanes: Methylosome protein 50 antibody at 6ug/ml Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 37, 30 kDa Observed band size: 37 kDa

WDR77 Antibody

CSB-PA859030ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetWDR77
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Overview

  • Supplier
    Cusabio
  • Product Name
    WDR77 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79084
  • Target name
    WDR77
  • Target description
    WD repeat domain 77
  • Target synonyms
    androgen receptor cofactor p44; HKMT1069; MEP50; MEP-50; methylosome protein 50; Nbla10071; p44; p44/Mep50; testis tissue sperm-binding protein Li 44a; WD repeat-containing protein 77
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQA1
  • Protein Name
    Methylosome protein 50
  • Scientific Description
    Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of WDR77 using anti-WDR77 antibody (A04894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR77 antigen affinity purified polyclonal antibody (Catalog # A04894-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR77 at approximately 42 kDa. The expected band size for WDR77 is at 42 kDa.
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