Western blot analysis is shown using GeneTex Affinity Purified anti-Human WHIP antibody (GTX24731) to detect Human WHIP present in a HEK293 whole cell lysate. ~30mg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected. The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WHIP or post translational modification of the WHIP protein. The blot was incubated with a 1:200 dilution of the antibody at room temperature for 2 h followed by detection using infrared labeled Goat-a-Rabbit IgG [H&L] MX10 diluted 1:5,000 for 45 min. The fluorescence image was captured using the OdysseyR Infrared Imaging System developed by LI-COR.