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ProductsBack/Wingless Type MMTV Integration Site Family, Member 2B (WNT2B) Polyclonal Antibody

Wingless Type MMTV Integration Site Family, Member 2B (WNT2B) Polyclonal Antibody

CAU21402
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetWNT2B
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Overview

  • Supplier
    Biomatik
  • Product Name
    Wingless Type MMTV Integration Site Family, Member 2B (WNT2B) Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Applications Supplier
    WB; IHC; ICC; IP.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7482
  • Target name
    WNT2B
  • Target description
    Wnt family member 2B
  • Target synonyms
    protein Wnt-2b; wingless-type MMTV integration site family, member 13; wingless-type MMTV integration site family, member 2B; WNT13; XWNT2, Xenopus, homolog of
  • Host
    Rabbit
  • Protein IDQ93097
  • Protein Name
    Protein Wnt-2b
  • Scientific Description
    The Wingless Type MMTV Integration Site Family, Member 2B (WNT2B) Polyclonal Antibody (Species: Human) has been validated for the following applications: WB, IHC, ICC, IP.
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Wnt2b using anti-Wnt2b antibody (A04879-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human SW620 whole cell lysates, Lane 7: human SKOV3 whole cell lysates, Lane 8: rat PC-12 whole cell lysates, Lane 9: mouse Neuro-2a whole cell lysates, Lane 10: mouse ANA-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Wnt2b antigen affinity purified polyclonal antibody (Catalog # A04879-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Wnt2b at approximately 60KD. The expected band size for Wnt2b is at 60KD.
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